Principles of plant amylase determination Methods of plant amylase determination Applications of plant amylase determination Amylase determination Amylase is an important type of enzyme in plants. It plays a vital role in plant growth and development, starch metabolism and stress resistance. With the continuous development of biotechnology, the determination technology of plant amylase is becoming increasingly mature. This article will introduce in detail the principles, methods and applications of plant amylase determination in agriculture, Application prospects in food and medicine and other fields. Principle of Plant Amylase Determination Plant Amylase Determination mainly evaluates its content by detecting amylase activity. Amylase activity refers to the ability of amylase to catalyze starch decomposition, which is usually expressed as the amount of starch decomposition per unit time. Plant amylase determination The principle is based on the specific hydrolysis effect of amylase on starch, and the amylase activity is calculated by detecting the hydrolysate. Method for determining plant amylase 1. Enzyme-linked immunosorbent assay (ELISA): ELISA is a commonly used method for determining plant amylase. It has the advantages of high sensitivity, strong specificity, and simple operation. This method detects amylase and substrate binding to form a complex to indirectly measure amylase activity.
2. Enzyme colorimetric method: Enzyme colorimetric method is another commonly used method for determining plant amylase. Its principle is that amylase catalyzes starch hydrolysis to produce glucose, and amylase activity is calculated by measuring the glucose concentration.
3. High performance liquid chromatography (HPLC): HPLC is a high-precision method for determining plant amylase. It is suitable for the determination of amylase activity in complex samples. This method determines amylase activity by separating and detecting amylase hydrolysate. Application of plant amylase determination 1. Agricultural field: Plant amylase determination can be used to evaluate plant stress resistance, growth and development, starch accumulation, etc. By measuring plant amylase activity, it can provide scientific basis for breeding, cultivation and pest control.
2. Food field: The determination of plant amylases is of great significance in food processing. During the starch extraction process, process parameters can be optimized and starch extraction rate can be improved by measuring amylase activity. Plant amylases can also be used for the development of food additives. and testing.
3. Pharmaceutical field: Plant amylases have broad application prospects in the pharmaceutical field. Amylases can be used as drug carriers to improve the bioavailability of drugs; amylases can also be used to treat diseases such as amylase deficiency. Prospects for plant amylase determination With the continuous development of biotechnology, plant amylase determination technology will become more mature and complete, and plant amylase determination will make breakthroughs in the following aspects: 1. Innovation of determination methods: Develop more sensitive, rapid, simple determination methods to improve determination accuracy.
2. Expansion of application fields: Apply plant amylase determination technology to more fields, such as bioenergy, biomaterials, etc.
3. Advances in data analysis technology: Combine big data, artificial intelligence and other technologies to deeply mine plant amylase measurement data to provide strong support for scientific research.
Plant amylase determination has wide application prospects in agriculture, food and medicine. With the continuous advancement of technology, plant amylase determination will provide strong support for research and production in related fields.