Aloe Tissue Culture and Rapid Propagation
Regarding the content about the propagation of this flower, specifically aloe tissue culture and rapid propagation, the details are as follows:
Aloe not only has certain ornamental value but can also be used in pharmaceuticals, food, cosmetics, and other aspects, possessing a wide range of practical uses. Its development and utilization are promising.
Aloe propagation usually adopts the division method or cutting method, with a low propagation coefficient and is limited by seasons. Using tissue culture to induce callus and cluster buds, then carrying out subculture expansion, a large number of seed sources can be obtained in a short period, and it can be produced all year round. This method especially shows its advantage when introducing new varieties.
I. Materials and Methods
1. Plant Name: Aloe
2. Material Category: Young Plant Stem Sections
3. Culture Conditions: Using MS medium as the basic culture medium, with the addition of: ① BA (Cytokinin) 0.5-3 mg/L (the same unit below) + NAA (Naphthylacetic Acid) 0.01-0.5; ② BA 0.5-1 + NAA 0.01 + GA3 (Gibberellin) 0.2-1; ③ IBA (Indole Acetic Acid) 0.2-0.4 + CCC (Chlormequat) 1; Culture temperature 23-27°C; Lighting 1500-2000 lux, with about 10 hours of light per day.
4. Experimental Method: Take the stem sections of young plants during the growing period, disinfect them, and on the ultra-clean workbench, separate the explants into 0.5-1 cm small sections, inoculate them into the medium ① for induction culture. After 2-3 weeks, the explants begin to form a large amount of callus and a small number of cluster buds; then transfer them to medium ②, and after a week, a large number of cluster buds begin to form. When the buds are 1.5-2 cm tall, separate them and transfer them to medium ②, and in three weeks, a complete plant can be formed, with a seedling height of 3-5 cm and root length of about 2 cm. If a large-scale propagation is needed, the callus can be subcultured and expanded in medium ①.
II. Establishment and Storage of Asexual Lines Aloe callus can be stored as a seed source at 5°C. Callus stored in a refrigerator at around 5°C for five months can still be normally induced to differentiate. After storage, the callus should be cultured in the culture room for three days to check for contamination before expanding the culture. There is basically no contamination, and the culture medium can be replaced for long-term storage.
III. Transplanting and Management of Test Tube Seedlings Test tube seedlings have poor adaptability to the external environment and must be acclimatized before being planted. The method is to open the bottle cap before taking out the seedlings, keep them in the culture room for 2-3 days for acclimatization, avoid damaging the roots when taking the seedlings, and wash the attached culture medium in water at the same room temperature to prevent root rot. The substrate can be garden soil mixed with an appropriate amount of vermiculite or all vermiculite. After planting, water with nutrient solution, cover with plastic film to keep warm and moist, open for ventilation for 30 minutes each day, and after four weeks, they can be transplanted and planted.
The above is the complete introduction to aloe tissue culture and rapid propagation, for everyone's learning and understanding!